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cap structure analog  (New England Biolabs)


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    Structured Review

    New England Biolabs cap structure analog
    Cap Structure Analog, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m7g/m7G(5)ppp(5)A+RNA+Cap+Structure+Analog/pm42862866-319-19-22
    Average 94 stars, based on 100 article reviews
    cap structure analog - by Bioz Stars, 2026-11
    94/100 stars

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    other:

    Article Title: eIF4E-independent translation is largely eIF3d-dependent
    Article Snippet: To clone the 5′-UTRs of interest into this bi-cistronic construct, the 5′-UTRs were amplified with oligos caring EcoR1-Eco32I sites and cloned via these sites into the intercistronic region.

    In Vitro:

    Article Title: An efficient mRNA delivery system for genome editing in plants
    Article Snippet: Then it was purified with a Monarch RNA Cleanup Kit (T2040; NEB, Ipswich, England). mRNA concentrations were determined on a Nanodrop 2000 (Thermo Fisher Scientific, Waltham, USA). .. In vitro transcribed mRNA was then m7G‐capped using the Vaccinia Capping System (M2080S; NEB, Ipswich, England). ..

    Article Title: ADP-ribosylation of RNA in mammalian cells is mediated by TRPT1 and multiple PARPs.
    Article Snippet: .. The in vitro transcribed mRNA was poly(A) tailed, purified and m7G-capped using the Vaccinia Capping System (New England Biolabs) or ADP-ribosylated using human TRPT1 after dephosphorylation using RppH to produce monophosphorylated RNA suitable for ADP-ribosylation. .. These mRNAs were then used in in vitro translation assays using the PURExpress®in vitro protein synthesis kit (New England Biolabs) according to the manufacturer’s protocol with 2 g of m7G-capped, ADPr-capped or non-capped GLuc reporter mRNA.

    Purification:

    Article Title: Supporting information for: Structure-first identification of RNA elements that regulate dengue virus genome architecture and replication
    Article Snippet: .. RNAs were transcribed from XbaI-linearized plasmids (MEGAscript T7 Transcription kit; Thermo Fisher Scientific), purified (RNeasy MinElute columns; Qiagen), and capped with m7G (Vaccinia capping system; NEB). ..

    Article Title: Structure-first identification of conserved RNA elements that regulate dengue virus genome architecture and replication
    Article Snippet: Mutant replicon plasmid sequences were confirmed by both Sanger and Illumina sequencing. .. DENV replicon RNAs were transcribed from XbaI-linearized plasmids (MEGAscript T7 Transcription kit, Thermo Fisher Scientific), purified (RNeasy MinElute columns; Qiagen), capped with m7G (Vaccinia capping system, NEB), and purified again (RNeasy MinElute columns; Qiagen). ..

    Article Title: ADP-ribosylation of RNA in mammalian cells is mediated by TRPT1 and multiple PARPs.
    Article Snippet: .. The in vitro transcribed mRNA was poly(A) tailed, purified and m7G-capped using the Vaccinia Capping System (New England Biolabs) or ADP-ribosylated using human TRPT1 after dephosphorylation using RppH to produce monophosphorylated RNA suitable for ADP-ribosylation. .. These mRNAs were then used in in vitro translation assays using the PURExpress®in vitro protein synthesis kit (New England Biolabs) according to the manufacturer’s protocol with 2 g of m7G-capped, ADPr-capped or non-capped GLuc reporter mRNA.

    Article Title: Supporting information for: Structure-first identification of RNA elements that regulate dengue virus genome architecture and replication
    Article Snippet: Mutant replicon plasmid sequences were confirmed by both Sanger and Illumina sequencing. .. DENV replicon RNAs were transcribed from XbaI-linearized plasmids (MEGAscript T7 Transcription kit, Thermo Fisher Scientific), purified (RNeasy MinElute columns; Qiagen), capped with m7G (Vaccinia capping system, NEB), and purified again (RNeasy MinElute columns; Qiagen). ..

    De-Phosphorylation Assay:

    Article Title: ADP-ribosylation of RNA in mammalian cells is mediated by TRPT1 and multiple PARPs.
    Article Snippet: .. The in vitro transcribed mRNA was poly(A) tailed, purified and m7G-capped using the Vaccinia Capping System (New England Biolabs) or ADP-ribosylated using human TRPT1 after dephosphorylation using RppH to produce monophosphorylated RNA suitable for ADP-ribosylation. .. These mRNAs were then used in in vitro translation assays using the PURExpress®in vitro protein synthesis kit (New England Biolabs) according to the manufacturer’s protocol with 2 g of m7G-capped, ADPr-capped or non-capped GLuc reporter mRNA.



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